Primordial Germ Cell Transplantation for CRISPR/Cas9-based Leapfrogging in <em>Xenopus</em>
نویسندگان
چکیده
منابع مشابه
Leapfrogging: primordial germ cell transplantation permits recovery of CRISPR/Cas9-induced mutations in essential genes.
CRISPR/Cas9 genome editing is revolutionizing genetic loss-of-function analysis but technical limitations remain that slow progress when creating mutant lines. First, in conventional genetic breeding schemes, mosaic founder animals carrying mutant alleles are outcrossed to produce F1 heterozygotes. Phenotypic analysis occurs in the F2 generation following F1 intercrosses. Thus, mutant analyses ...
متن کاملPrimordial germ cell migration.
Mutational and antisense screens in Drosophila and zebrafish, and transcriptional profiling and time-lapse analysis in the mouse, have contributed greatly to our understanding of PGC development. In all three systems, the behavior of PGCs is controlled by growth factors which signal through G-protein coupled receptors and/or tyrosine kinase receptors. Additionally, regulated cell-cell and cell-...
متن کاملAn investigation of primordial germ cell development in embryo and larvae of Huso huso
In sturgeons, Primordial Germ Cells originate from the vegetal pole of the egg. They migrate toward genital ridges during developmental stages and finally differentiate into gametes (the only cells that transmit genetic information). In this study, the cells were isolated from the place of migration of primordial germ cells in Huso huso’s larvae during two stages before and after absorption of ...
متن کاملPrimordial germ cell development in zebrafish.
In sexually reproducing organisms, primordial germ cells (PGCs) give rise to gametes that are responsible for the development of a new organism in the next generation. These cells follow a characteristic developmental path that is manifested in specialized regulation of basic cell functions and behavior making them an attractive system for studying cell fate specification, differentiation and m...
متن کاملNuclear transplantation of male primordial germ cells in the mouse.
We examined the developmental ability of enucleated eggs receiving embryonic nuclei and male primordial germ cells (PGCs) in the mouse. Reconstituted eggs developed into the blastocyst stage only when an earlier 2-cell nucleus was transplanted (36%) but very rarely if the donor nucleus was derived from a later 2-cell, 8-cell, or inner cell mass of a blastocyst (0-3%). 54-100%, 11-67%, 6-43% and...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2018
ISSN: 1940-087X
DOI: 10.3791/56035